CST, 63722SF, Rab6 (D37C7) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying RAB6. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Rab6 (D37C7) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63722) is ready to ship. Product Usage Information This product is the carrier free version of product #9625. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9625 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rab6 (D37C7) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Rab6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Rab6 protein. Background Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12). Alternate Names GTP-binding protein RAB6A'; GTP-binding protein RAB6B; Oncogene RAB6; Rab GTPase; Rab-6; RAB6; RAB6, member RAS oncogene family; RAB6A; Rab6A variant 3; RAB6A, member RAS oncogene family; RAB6A'; RAB6B; Ras-related protein Rab-6A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit