CST, 63779S, Fibronectin/FN1 Antibody

Polyclonal Antibody for studying FN1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Fibronectin/FN1 Antibody (CST #63779) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Fibronectin/FN1 Antibody recognizes endogenous levels of total mouse or rat Fibronectin/FN1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp111 of mouse Fibronectin/FN1 protein. Antibodies are purified by peptide affinity chromatography. Background Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6). Alternate Names Anastellin; E330027I09; Fibronectin; fibronectin 1; FINC; FN; Fn-; Fn-1; Fn1; MGC117493 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 300 SOURCE: Rabbit