CST, 63815SF, PD-1 (Intracellular Domain) (D4W2J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PDCD1. Validated for WB,IHC,IHC,IF,F. Highly specific and rigorously validated in-house, PD-1 (Intracellular Domain) (D4W2J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63815) is ready to ship. Product Usage Information This product is the carrier free version of product #86163. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 86163 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PD-1 (Intracellular Domain) (D4W2J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PD-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein. Background The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10). Alternate Names CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; programmed cell death 1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52-65 Source/Isotype: Rabbit IgG