CST, 63862S, EXO1 (E6P2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EXO1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, EXO1 (E6P2B) Rabbit Monoclonal Antibody (CST #63862) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EXO1 (E6P2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total EXO1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro377 of human EXO1 protein. Background Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (1). In addition to its role in MMR, the nuclease EXO1 functions in DNA double-strand break repair, replication stress response, nucleotide excision repair (NER), and telomere maintenance (2). AMPK phosphorylates EXO1 at Ser746 in response to replication stress and subsequent calcium elevation to avoid replication fork resection (3). Mutations in the gene are associated with multiple human cancers (4). Alternate Names EXO1; EXOI; Exonuclease 1; Exonuclease I; HEX1; hExo1; hExoI; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG