CST, 63965S, CD11b/ITGAM (F2V1P) Mouse Monoclonal Antibody

Monoclonal Antibody for studying CD11b/ITGAM. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CD11b/ITGAM (F2V1P) Mouse Monoclonal Antibody (CST #63965) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD11b/ITGAM (F2V1P) Mouse Monoclonal Antibody recognizes endogenous levels of total CD11b/ITGAM protein. Species reactivity for immunofluorescence is human only. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro265 of human CD11b/ITGAM protein. Background Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4). Alternate Names antigen CD11b (p170); CD11 antigen-like family member B; CD11B; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; Integrin alpha-M; integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM; ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Mouse IgG1