CST, 63972S, BCOR (E6V3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCoR. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, BCOR (E6V3R) Rabbit Monoclonal Antibody (CST #63972) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity BCOR (E6V3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCOR protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCOR protein. Background BCL6 corepressor protein (BCOR) is a ubiquitously expressed transcriptional repressor that is recruited to target genes by BCL6 and other transcription factors (1). BCOR interacts with and recruits histone deacetylase (HDAC) and DNA methyltransferase (DNMT) proteins to facilitate localized histone deacetylation and DNA methylation, resulting in repression of gene expression (1,2). Mutations and translocations involving BCOR have been observed in acute myeloid leukemia, bone sarcoma, and medulloblastoma, and mutations in BCOR are known to cause X-linked Oculofaciocardiodental and Lenz microphthalmia syndromes (3-6). Alternate Names 5830466J11Rik; 8430401K06Rik; ANOP2; BCL-6 coreceptor; BCL-6 corepressor; BCL-6 interacting corepressor; BCL6 co-repressor; BCL6 corepressor; BCL6 interacting corepressor; BCOR; FLJ20285; FLJ38041; KIAA1575; MAA2; MCOPS2; MGC131961; MGC71031 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 210, 110 Source/Isotype: Rabbit IgG