CST, 64022S, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying FUT4. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #64022) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated). Background SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2). Alternate Names alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; FucT-IV; FUT4; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Mouse IgM