CST, 64038S, MYH7 (E3G8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYH7. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MYH7 (E3G8Q) Rabbit Monoclonal Antibody (CST #64038) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MYH7 (E3G8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYH7 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1932 of human MYH7 protein. Background Myosin heavy chain 7 (MYH7), also known as β-myosin heavy chain, is a type I slow-twitch fiber expressed in muscle fibers as well as heart ventricles and is essential for muscle contraction (1). MYH7 is composed of a globular head domain at the N-terminus, a hinge region, and an α-helical coil-coil rod domain (2). MYH7 forms a hexameric complex with two MYH7, two myosin light chain 2 (MYL2), and two myosin light chain 3 (MYL3) (3). The myosin-binding sites on actin filaments become exposed by the binding of Ca+, which leads to actin-myosin junction formation and the ability to glide along the actin base. The complexes become detached in the presence of ATP, and the next cycle of contraction is initiated when ATPase activity of the head domain hydrolyzes ATP (4). Mutations in the gene can lead to hypertrophic cardiomyopathy (HCM), dilated CM (DCM), and left ventricle non-compaction (LVNC) (5). Alternate Names beta-myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; CMD1S; CMH1; DKFZp451F047; MGC138376; MGC138378; MPD1; MYH7; MyHC-beta; MyHC-slow; MYHCB; myopathy, distal 1; myosin 7; myosin heavy chain (AA 1-96); Myosin heavy chain 7; myosin heavy chain beta-subunit; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; Myosin-7; myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 140-210 Source/Isotype: Rabbit IgG