CST, 64055SF, GABARAP (E1J4E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GABARAP. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, GABARAP (E1J4E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64055) is ready to ship. Product Usage Information This product is the carrier free version of product #13733. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13733 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GABARAP (E1J4E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GABARAP protein. This antibody does not cross-react with other GABARAP family members. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human GABARAP protein. Background GABA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11). Alternate Names ATG8A; epididymis secretory sperm binding protein; FLC3B; FLJ25768; GABA type A receptor-associated protein; GABA(A) receptor-associated protein; GABARAP; GABARAP-a; Gamma-aminobutyric acid receptor-associated protein; GBRAP; MGC120154; MGC120155; MM46 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14, 16 Source/Isotype: Rabbit IgG