CST, 64099S, LRP1 Antibody

Polyclonal Antibody for studying LRP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LRP1 Antibody (CST #64099) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LRP1 Antibody recognizes endogenous levels of total LRP1 β subunit. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LRP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Low density lipoprotein receptor related protein 1 (LRP1) is a type I transmembrane receptor that mediates the endocytosis of various ligands (1). LRP1 plays important roles in lipid homeostasis, signaling transduction, embryonic development, and glucose metabolism (2-6). In addition, LRP1 regulates APP processing and facilitates the clearance of beta-amyloid (7-9). This finding makes LRP1 a potential therapeutic target for Alzheimer's disease. LRP1 preprotein is proteolytically processed by furin to generate a 515 kDa extracellular α subunit and a membrane-anchored 85 kDa β subunit, which together form the mature receptor (10). Alternate Names A2MR; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; APR; CD91; FLJ16451; IGFBP-3R; IGFBP3R; IGFBP3R1; KPA; LDL receptor related protein 1; low density lipoprotein receptor-related protein 1; Low-density lipoprotein receptor-related protein 1 515 kDa subunit; Low-density lipoprotein receptor-related protein 1 85 kDa subunit; Low-density lipoprotein receptor-related protein 1 intracellular domain; LRP; LRP-1; LRP-515; LRP-85; LRP1; LRP1A; LRPICD; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; TGFBR5; type V tgf-beta receptor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit