CST, 64131S, XCL1/XCL2 (E9D3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XCL1. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, XCL1/XCL2 (E9D3H) Rabbit Monoclonal Antibody (CST #64131) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79671 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity XCL1/XCL2 (E9D3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total XCL1/XCL2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly101 of human XCL1 protein. Background XCL1 (Lymphotactin, SCM-1 alpha, Chemokine (C motif) ligand 1) and XCL2 (SCM-1 beta, Chemokine (C motif) ligand 2) comprise the C chemokine family, lacking two of the four invariant cysteines normally found on chemokines (1,4). Their sequences differ by 2 amino acids and they are primarily expressed by CD8 T cells and NK cells (1-5). XCL1 and XCL2 act as ligands for the chemokine receptor XCR1 (1-5). XCR1-positive dendritic cells cross-present antigens to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (6,7). In addition, in mouse models, the XCL1-XCR1 signaling axis was shown to be involved in the formation of self-tolerance through the development of T cells within the thymus (8). Alternate Names ATAC; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM-1; SCM-1-alpha; SCM-1a; SCM1; SCM1A; SCYC1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; X-C motif chemokine ligand 1; XC chemokine ligand 1; XCL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG