CST, 64162SF, NUT (C52B1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NUT1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, NUT (C52B1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64162) is ready to ship. Product Usage Information This product is the carrier-free version of product #3625. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3625 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NUT (C52B1) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total NUT protein. This antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC). Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to human NUT protein. Background Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2). Alternate Names C15orf55; DKFZp434O192; FAM22H; MGC138683; MGC138684; Nuclear protein in testis; NUT; NUT family member 1; NUT midline carcinoma family member 1; NUTM1; Protein NUT Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG