CST, 64266SF, PLVAP (E3X9D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PLVAP. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, PLVAP (E3X9D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64266) is ready to ship. Product Usage Information This product is the carrier free version of product #38238. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 38238 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PLVAP (E3X9D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PLVAP protein. Non-specific staining was observed in gall bladder by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PLVAP protein. Background PLVAP (plasmalemma vesicle-associated protein) is an endothelial cell (EC)-specific type II membrane protein. It forms diaphragms that bridge the opening of caveolae, fenestrae, and trans-endothelial channels and plays a role in regulating vascular permeability. PLVAP expression is restricted to a subset of ECs in the normal microvasculature, but it is not expressed in the ECs of large vessels, with the exception of the endocardial lining of the heart chambers (1). While PLVAP is expressed in ECs in the brain and retina during embryogenesis and postnatal development, it is not expressed in mature endothelium that forms blood-brain barrier (BBB) and blood-retinal barrier (BRB). However, under pathological conditions associated with barrier disruption such as brain ischemia, cancer, and diabetic retinopathy, PLVAP expression is induced in endothelium of BBB and BRB (2). PLVAP was previously considered as an EC marker of the vascular system, its expression is now reported in lymphatic ECs (LECs) in lymph nodes and it may play a role in regulating the parenchymal entry of lymphocytes and soluble antigens (3,4). Alternate Names DIAR10; FELS; Fenestrated endothelial-linked structure protein; fenestrated-endothelial linked structure protein; gp68; plasmalemma vesicle associated protein; Plasmalemma vesicle protein 1; Plasmalemma vesicle-associated protein; PLVAP; PV-1; PV-1 protein; PV1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70, 140 Source/Isotype: Rabbit IgG