CST, 64274T, Stathmin-4 (E9F1Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STMN4. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Stathmin-4 (E9F1Y) Rabbit Monoclonal Antibody (CST #64274) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Stathmin-4 (E9F1Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total Stathmin-4 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Stathmin-4 protein. Background Stathmin is a family of microtubule-destabilizing phosphoproteins that play an important role in regulation of the microtubule cytoskeleton (1). The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules, regulating cellular processes, such as cell cycle and neuronal axon development (2). Stathmin family members include stathmin, stathmin-2, stathmin-3, and stathmin-4. Interestingly, stathmin-2 protein levels correlate with decreased TDP43 expression, an mRNA-binding protein that mislocalizes and aggregates in patients with the motor neuron disease, amyotrophic lateral sclerosis (ALS) (3). Interestingly, TDP43 directly regulates stathmin-2 expression by regulating RNA splicing, suggesting that altered stathmin-2 expression might serve as a biomarker for disease progression or a therapeutic target. Altered Stathmin-4 expression in oligodendrocytes has been identified in Alzheimer's disease (4). Alternate Names MGC111012; RB3; stathmin 4; Stathmin-4; stathmin-like 4; Stathmin-like protein B3; stathmin-like-protein RB3; STMN4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22, 25 Source/Isotype: Rabbit IgG