CST, 64306SF, OMA1 (D4J7K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying OMA1. Validated for Western Blotting. Highly specific and rigorously validated in-house, OMA1 (D4J7K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64306) is ready to ship. Product Usage Information This product is the carrier free version of product #95473. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 95473 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity OMA1 (D4J7K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total OMA1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu504 of human OMA1 protein. Background Mitochondria continuously divide and fuse. This dynamic process is highly regulated in response to various physiological cues (1,2). The GTPase OPA1 mediates the fusion of the mitochondrial inner membrane. Constitutive proteolytic processes mediated by OMA1 (S1 site) and YME1L (S2 site) convert long isoforms (L-OPA1) into short isforms (S-OPA1). The balance between L-OPA1 and S-OPA1 is required to maintain a normal morphology of mitochondria (3,4). OMA1 is synthesized as a precursor and processed into a mature form (5,6). OMA1 is constitutively active and cleaves L-OPA1 at the S1 site. However, various stress stimuli can further activate OMA1 and result in the rapid and complete conversion of L-OPA1 into S-OPA1, which inhibits fusion and causes mitochondrial fragmentation (6). Alternate Names 2010001O09Rik; DAB1; FLJ33782; Metalloendopeptidase OMA1, mitochondrial; metalloprotease related protein 1; Metalloprotease-related protein 1; MPRP-1; MPRP1; OMA1; OMA1 homolog, zinc metallopeptidase; OMA1 homolog, zinc metallopeptidase (S. cerevisiae); OMA1 zinc metallopeptidase; OMA1 zinc metallopeptidase homolog; overlapping activity with M-AAA protease; Overlapping with the m-AAA protease 1 homolog; peptidase; YKR087C; zinc metallopeptidase OMA1; ZMPOMA1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG