CST, 64329S, PRDX6 (D9J9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRDX6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PRDX6 (D9J9H) Rabbit Monoclonal Antibody (CST #64329) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PRDX6 (D9J9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRDX6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PRDX6 protein. Background Peroxiredoxin 6 (PRDX6) belongs to an antioxidant enzyme family of non-seleno peroxidases. PRDX6 is a unique member of the PRDX family, exhibiting both glutathione peroxidase and phospholipase A2 activities (1,2). PRDX6 regulates phospholipid turnover in addition to protecting cells against oxidative injury. PRDX6 is expressed in all major organs, with a particularly high level in lung, where it regulates lung surfactant phospholipid synthesis and turnover (3-5). Research studies have shown that PRDX6 is aberrantly expressed in various cancers, and can promote cancer cell metastasis and invasion (6,7). Elevated expression of PRDX6 and related PRDX family members has also been shown to contribute to drug resistance in cancer cells (8,9). PRDX6 is also expressed in neutrophils, where it has been shown to activate NADPH oxidase (NOX2) (10-12). Alternate Names 1-Cys; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; epididymis secretory sperm binding protein Li 128m; Glutathione-dependent peroxiredoxin; HEL-S-128m; KIAA0106; Liver 2D page spot 40; MGC46173; Non-selenium glutathione peroxidase; NSGPx; p29; peroxiredoxin 6; Peroxiredoxin-6; PRDX6; PRX; Red blood cells page spot 12 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG