CST, 6434S, COUP-TFII (D16C4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NR2F2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, COUP-TFII (D16C4) Rabbit Monoclonal Antibody (CST #6434) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity COUP-TFII (D16C4) Rabbit Monoclonal Antibody detects endogenous levels of total COUP-TFII protein. This antibody does not cross-react with COUP-TFI protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln158 of human COUP-TFII protein. Background Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family (1). COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes (1-3). COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression (4,5). COUP-TFI is involved in neuronal development, tissue patterning, and differentiation (6-8). COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment (9-12). Alternate Names ADP-ribosylation factor related protein 1; Apolipoprotein A-I regulatory protein 1; apolipoprotein AI regulatory protein 1; ARP-1; ARP1; chicken ovalbumin upstream promoter transcription factor 2; chicken ovalbumin upstream promoter-transcription factor I; CHTD4; COT2; COUP transcription factor 2; COUP transcription factor II; COUP-TF II; COUP-TF2; COUPTF2; COUPTFB; COUPTFII; MGC117452; NF-E3; NR2F2; Nuclear receptor subfamily 2 group F member 2; nuclear receptor subfamily 2, group F, member 2; SRXX5; SVP40; TFCOUP2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG