CST, 64414S, Transketolase (E7O4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Transketolase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Transketolase (E7O4M) Rabbit Monoclonal Antibody (CST #64414) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Transketolase (E7O4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total transketolase protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val296 of human transketolase protein. Background Transketolase (TKT) is a homodimer in the pentose phosphate pathway (PPP) that catalyzes the interketol transfer between ketoses and aldoses (1,2). This enzyme, along with transaldolase, connects the nonoxidative branch of the PPP with glycolysis (1-3). Several regions of TKT are evolutionarily conserved from gram-negative bacteria to mammals (3). There is evidence that hypoxic (4) and non-hypoxic induction of HIF1-α (5) increases the expression of TKT. Because cancer cells rely on TKT in altered cell metabolism for nucleic acid synthesis, work has been done to develop inhibitors of TKT as novel cancer treatments (5-8). Alternate Names epididymis luminal protein 107; epididymis secretory protein Li 48; FLJ34765; HEL-S-48; HEL107; SDDHD; TK; TKT; TKT1; Transketolase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG