CST, 64468S, RPS4Y1 (F7V5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RPS4Y1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, RPS4Y1 (F7V5G) Rabbit Monoclonal Antibody (CST #64468) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RPS4Y1 (F7V5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total RPS4Y1 protein. This antibody detects an approximately 70 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val156 of human RPS4Y1 protein. Background Ribosomal protein S4, Y-linked 1 (RPS4Y1) is a structural constituent of the 40S small subunit of the cytoplasmic ribosome, the fundamental machinery orchestrating protein synthesis within eukaryotic cells. Integral to the ribosome's architecture, RPS4Y1 plays a crucial role in the proper assembly and stability of the 40S subunit, and contributes to the fidelity and efficiency of messenger RNA (mRNA) translation (1-3). The human genome harbors paralogous genes encoding ribosomal protein S4, including RPS4X located on the X chromosome, alongside RPS4Y1 and RPS4Y2 on the Y chromosome. Being a Y-linked gene, exhibits primary expression in male cells (1,4). Notably, haploinsufficiency of ribosomal protein genes, encompassing those residing on sex chromosomes, has been implicated in the pathogenesis of diverse developmental disorders (5). The somatic loss of the Y chromosome (LoY), observed in cells of aging males, has also been intriguingly documented in several tumor types, prompting speculation regarding the roles of RPS4Y1 and other Y-linked loci in the pathology of these malignancies (4-6). Certain studies have proposed a potential involvement of RPS4X and RPS4Y1 in Turner syndrome, a genetic condition affecting females characterized by a missing or structurally altered X chromosome. Moreover, aberrant regulation of RPS4Y1 expression has been noted in specific pathological states, such as preeclampsia, where it may influence trophoblast cell migration and invasion throughsignaling cascade modulation, including Stat3 (1,3). The Y-linked genomic location of RPS4Y1 and the potential for differential expression relative to its X-linked counterpart led to interest in RPS4Y1 as a potential marker for identifying male cells (6,7). Alternate Names 40S ribosomal protein S4, Y; 40S ribosomal protein S4, Y isoform 1; MGC119100; MGC5070; ribosomal protein S4 Y-linked 1; ribosomal protein S4, Y-linked; ribosomal protein S4, Y-linked 1; ribosomal protein S4Y; RPS4Y; RPS4Y1; RS4Y1; S4; Small ribosomal subunit protein eS4; Small ribosomal subunit protein eS4, Y isoform 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG