CST, 64471SF, p63 (D9L7L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying p63. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, p63 (D9L7L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64471) is ready to ship. Product Usage Information This product is the carrier-free version of product #39692. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 39692 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity p63 (D9L7L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total p63. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize both full-length (TA) p63 as well as DeltaN p63 isoforms that contain exon 4, such as alpha, beta, and gamma. This antibody will not detect DeltaNp73L (Q9H3D4-10/NM_001329146.1). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn118 of human p63 protein. Background The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the gene in squamous cell carcinomas of the lung, head, and neck (2,3). The gene contains an alternative transcription initiation site that yields a truncated ΔNp63 lacking the transactivation domain, and alternative splicing at the carboxy terminus yields the α, β, and γ isoforms (3,4). Alternate Names AIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DeltaNp63; EEC3; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p53CP; P63; P73H; P73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; Tumor protein 63; tumor protein p53-competing protein; tumor protein p53-like; tumor protein p63; tumor protein p63 deltaN isoform delta; Tumor protein p73-like Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG