CST, 6447S, DR1 Antibody

Polyclonal Antibody for studying DR1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DR1 Antibody (CST #6447) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DR1 Antibody recognizes endogenous levels of total DR1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human DR1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Down-regulator of transcription 1 (DR1), also known as negative cofactor 2-β (NC2-β), forms a heterodimer with DR1 associated protein 1 (DRAP1)/NC2-α and acts as a negative regulator of RNA polymerase II and III (RNAPII and III) transcription (1-5). DR1 activity is thought to be important for modulating the switch between basal transcription activity and transcription activator driven transcription (2,6,7). DR1 interaction with TATA binding protein (TBP) blocks the association of general transcription factors TFIIA and TFIIB with TBP and disrupts the formation of the RNAPII transcription initiation complex (1,8,9). RNAPIII driven transcription is also inhibited by DR1 interaction with TBP. DR1 disrupts the interaction of TBP with the TFIIB related factor (BRF)/RNAPIII B-related factor, inhibiting transcription initiation by the RNAPIII machinery (4). Alternate Names Down-regulator of transcription 1; down-regulator of transcription 1, TBP-binding (negative cofactor 2); DR1; NC2; NC2-beta; NC2B; NCB2; negative cofactor 2; Negative cofactor 2-beta; Protein Dr1; TATA-binding protein-associated phosphoprotein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 19 SOURCE: Rabbit