CST, 64502S, HTRA1/PRSS11 (E6Y8E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HTRA1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, HTRA1/PRSS11 (E6Y8E) Rabbit Monoclonal Antibody (CST #64502) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HTRA1/PRSS11 (E6Y8E) Rabbit Monoclonal Antibody recognizes endogenous levels of total HTRA1/PRSS11 protein. This antibody detects both the full-length protein and the autolytically processed serine protease domain. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the serine protease and PDZ domains of human HTRA1/PRSS11 protein. The antigen has been further characterized as corresponding to residues surrounding Val209 of human HTRA1/PRSS11 protein. Background High-temperature requirement A serine peptidase 1 (HTRA1, PRSS11) is a trypsin-like serine protease involved in neuronal maturation, protein degradation, apoptosis, and the response to cellular stress. HTRA family members contain N-terminal IGFBP and Kazal-like domains, a serine protease domain, and a C-terminal PDZ domain (1). HTRA1 can be secreted and autocatalyzed (2) or retained in the cell and processed to shorter cytoplasmic- and nuclear-localized forms (3). Ser328Ala mutants lack both proteolytic and autocatalytic activity (2). HTRA1 regulates the extracellular matrix (ECM) by degrading aggrecan, collagen, decorin, fibromodulin, and fibronectin (4,5), while also breaking down signaling molecules such as TGF-β family members (6,7) and FGF8 (8). In the brain, HTRA1 degrades β-Amyloid and aggregated fibrillar tau (9,10). Elevated HTRA1 can contribute to osteoarthritis (5,11) and age-related macular degeneration (12). HTRA1 loss induces EMT and breast cancer cell invasion (13). Alternate Names ARMD7; CADASIL2; CARASIL; High-temperature requirement A serine peptidase 1; HTRA; HtrA serine peptidase 1; HTRA1; IGFBP5-protease; L56; ORF480; protease, serine, 11 (IGF binding); PRSS11; Serine protease 11; Serine protease HTRA1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40-50, 25-30 Source/Isotype: Rabbit IgG