CST, 64558S, Metavinculin (F3R2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Metavinculin. Validated for WB,WB,IP,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Metavinculin (F3R2D) Rabbit Monoclonal Antibody (CST #64558) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Metavinculin (F3R2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total metavinculin protein. Based on the sequence of the peptide antigen, this antibody is not expected to detect vinculin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro943 of human metavinculin protein. Background Vinculin is a cytoskeletal protein localized to cell-matrix and cell-cell adhesion complexes with roles in cell adhesion, migration, and signal transduction (1). Metavinculin is a vinculin isoform resulting from alternative splicing containing a 68-residue insert (2). Â Metavinculin is expressed in cardiac and smooth muscle (3), and mutations in the metavinculin insert are associated with various cardiomyopathies (4). Metavinculin protein functions in the cytoskeleton with roles in actin bundling, focal adhesion formation, and cell adhesion and migration (5-7). Alternate Names CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; meta-vinculin; Metavinculin; MV; MVCL; VCL; VINC; Vinculin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG