CST, 64565W2, GATA-1 (D52H6) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 532 Conjugate)

Monoclonal Antibody for studying GATA1. Highly specific and rigorously validated in-house, GATA-1 (D52H6) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 532 Conjugate) (CST #64565) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity GATA-1 (D52H6) XP Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu13 of human GATA-1. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in the corresponding gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9). Alternate Names ERYF1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA1; GF-1; GF1; globin transcription factor 1; NF-E1; NF-E1 DNA-binding protein; NFE1; nuclear factor, erythroid 1; transcription factor GATA1; XLANP; XLTDA; XLTT Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG