CST, 64706S, FAP (F1A4G) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying FAP. Validated for Immunohistochemistry (Paraffin),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, FAP (F1A4G) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #64706) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 - 1:800 Flow Cytometry (Live): 1:2000 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Live) Specificity / Sensitivity FAP (F1A4G) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human FAP protein. Background Fibroblast activation protein/seprase (FAP) is a cell surface serine integral membrane protease with roles in tissue remodeling during development and wound healing, as well as in cancer and fibrotic disease. FAP has both dipeptidyl peptidase and endopeptidase activities, and cleaves at N-terminal Xaa-Pro sequences. FAP is highly expressed in tumor stroma, and contributes to migration and invasion of tumor cells, as well as that of endothelial cells, into the extracellular matrix. FAP is a useful marker of tumorigenic stroma and of cancer-associated fibroblasts (CAFs) and fibrosis (1,2). Alternate Names 170 kDa melanoma membrane-bound gelatinase; Antiplasmin-cleaving enzyme FAP, soluble form; APCE; Dipeptidyl peptidase FAP; DKFZp686G13158; DPPIV; FAP; FAPA; FAPalpha; Fibroblast activation protein alpha; fibroblast activation protein, alpha; fibroblast activation protein, alpha subunit; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; SEPR; Seprase; Serine integral membrane protease; SIMP; Surface-expressed protease Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG