CST, 64718S, MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MLANA. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody (CST #64718) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73236 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total MLANA/MART-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLANA/MART-1 protein. Background MLANA, also known as MART-1, is a member of a melanocyte lineage-specific family of proteins. It is expressed in melanocytes, retinal pigment epithelium, and melanoma cells. Its function is not entirely understood, but it is believed to be involved in the stability of GPR143, as well as the stability, trafficking, and processing of PMEL; both proteins are involved in the formation of stage II melanosomes (1). In melanosomes, MLANA is specifically located in the trans-Golgi network, however conformational changes to the protein or a sub-population of the protein causes it to localize back to the ER and small endosomal vesicles (2). In the context of melanoma cells, the conformational change is thought to be caused by aberrant exposure of epitopes, which are recognized by cytolytic T-lymphocytes (3). MLANA may be useful as a marker of metastatic melanoma (4). MHC-II restricted phospho-MLANA peptides, which are recognized by CD4 cells, are being investigated as potential candidates for cancer immunotherapy (5). Alternate Names Antigen LB39-AA; Antigen SK29-AA; epididymis secretory sperm binding protein; MAR1; MART-1; MART1; melan-A; Melanoma antigen recognized by T-cells 1; MLANA; Protein Melan-A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG