CST, 64733S, MED17 (E3V6Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MED17. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MED17 (E3V6Y) Rabbit Monoclonal Antibody (CST #64733) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MED17 (E3V6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total MED17 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly272 of human MED17 protein. Background The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10). MED17, along with MED6, 8, 11, 18, 20, and 22, make up the head module of the Mediator complex (11). MED17 is critical for head module assembly as it acts as a scaffold to recruit MED11 and MED22 (12). Phosphorylation of MED17 has been shown as a regulatory mechanism in lipogenesis and NF-κB signaling (13,14). Alternate Names Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6 (77kD); cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; CRSP6; CRSP77; DRIP77; DRIP80; epididymis secretory sperm binding protein; FLJ10812; MED17; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; SRB4; Thyroid hormone receptor-associated protein complex 80 kDa component; thyroid hormone receptor-associated protein, 80-KD subunit; Transcriptional coactivator CRSP77; TRAP80; vitamin D receptor interacting protein 80-kD; Vitamin D3 receptor-interacting protein complex 80 kDa component Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG