CST, 64801S, EphA7 (D1C3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EphA7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, EphA7 (D1C3K) Rabbit Monoclonal Antibody (CST #64801) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EphA7 (D1C3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total EphA7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu914 of human EphA7 protein. Background The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). The EphA7 receptor preferentially binds ephrin-A5 as a ligand. This ligand-receptor interaction stimulates EphA7 signaling and induces apoptotic cell death through TNFR1 and caspase-8 pathway (6,7). EphA7 plays a critical role in organ development during neural tube closure, cortical dendritic development and spine maturation as well as urine tract insertion (8-10). Secreted EphA7 has been shown to promote somatic cell reprogramming through ERK activity reduction (11). Silencing of the secreted form of EphA7 is associated with germinal center B cell lymhomas. The secreted form of EphA7 has been proposed as a soluble tumor suppresor in lymphoma (12-14). Alternate Names EHK-3; EHK3; EK11; EPH homology kinase 3; Eph homology kinase-3; EPH receptor A7; EPH-like kinase 11; EPHA7; ephrin receptor EphA7; Ephrin type-A receptor 7; HEK11; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG