CST, 64875SF, SHP-1 (E1U6R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SHP-1. Validated for WB,IF,IF,F. Highly specific and rigorously validated in-house, SHP-1 (E1U6R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #64875) is ready to ship. Product Usage Information This product is the carrier free version of product #26516. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 26516 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SHP-1 (E1U6R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SHP-1 protein. This antibody non-specifically labels the cytoplasm in fixed frozen mouse pancreas by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human SHP-1 protein. Background SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EPORs) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6). Alternate Names HCP; HCPH; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; protein tyrosine phosphatase non-receptor type 6; protein tyrosine phosphatase, non-receptor type 6; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1; Tyrosine-protein phosphatase non-receptor type 6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG