CST, 64933S, ZCCHC4 Antibody

Polyclonal Antibody for studying ZCCHC4. Validated for Western Blotting. Highly specific and rigorously validated in-house, ZCCHC4 Antibody (CST #64933) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ZCCHC4 Antibody recognizes endogenous levels of total ZCCHC4 protein. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZCCHC4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background N6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. Zinc finger CCHC domain-containing protein 4 (ZCCHC4) is an rRNA m6A methyltransferase that specifically carries out m6A methylation of 28S rRNA at position 4220 (1). ZCCHC4 is highly conserved among multicellular organisms, and knockout of ZCCHC4 ablates m6A methylation exclusively at position 4420 on 28s rRNA, suggesting that this is an evolutionarily conserved function (1,2). Knockout of ZCCHC4, and consequently the m6A 4220 modification, has been reported to reduce overall protein translation and inhibit cell proliferation, while others have reported that this knockout had no effect on cell proliferation nor rRNA production (1,2). ZCCHC4 has been found to be overexpressed in hepatocellular carcinoma, and ZCCHC4 knockout cells showed reduced tumor growth in a xenograft mouse model (1). Interestingly, X-ray crystallography reveals that ZCCHC4 contains an autoinhibitory regulatory loop that blocks its catalytic site, a unique feature amongst m6A RNA methyltransferases currently identified (3). Alternate Names DHHC domain-containing zinc finger protein; HSPC052; MGC21108; rRNA N6-adenosine-methyltransferase ZCCHC4; ZCCHC4; ZCHC4; ZGRF4; Zinc finger CCHC domain-containing protein 4; zinc finger CCHC-type containing 4; zinc finger, CCHC domain containing 4; zinc finger, GRF-type containing 4 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 51 SOURCE: Rabbit