CST, 64953T, VISTA (D1L2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VISTA. Validated for WB,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, VISTA (D1L2G) Rabbit Monoclonal Antibody (CST #64953) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 82119 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity VISTA (D1L2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total VISTA protein. Non-specific staining was observed in kidney and prostate epithelium by immunohistochemistry. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VISTA protein. Background VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7). Alternate Names B7-H5; B7H5; C10orf54; DD1alpha; Death Domain1alpha; Dies1; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; Sisp-1; SISP1; stress induced secreted protein 1; Stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation; VISTA; VSIR Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 45-65 Source/Isotype: Rabbit IgG