CST, 64995S, MHC Class I (E8E7N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying H2-D1 mouse. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MHC Class I (E8E7N) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #64995) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity MHC Class I (E8E7N) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu254 of mouse H2-D1 protein. Background The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8 T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4 T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2). Alternate Names H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H-2D cell surface glycoprotein; H-2D(B); H2-D; H2-D1; H2-DC1-beta transplantation antigen protein; H2-K1; HA11; histocompatibility 2, D region locus 1; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG