CST, 64996S, SAFB1 (F5Z6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SAFB1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SAFB1 (F5Z6A) Rabbit Monoclonal Antibody (CST #64996) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SAFB1 (F5Z6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAFB1 protein. This antibody does not cross-react with SAFB2 protein. This antibody detects a 75 kDa band of unknown origin in mouse and rat. Reactivity by immunofluorescence is human only. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala909 of human SAFB1 protein. Background Scaffold attachment factor B1 and B2 (SAFB1/B2) are homologs that bind to scaffold or matrix attachment region DNA elements (S/MAR DNA) (1,2). SAFB1 and SAFB2 bind to estrogen receptor to inhibit its activity, an action enhanced by tamoxifen treatment and opposed by histone deacetylase activity (2-4). SAFB1 and SAFB2 contain an RNA recognition motif and can interact with various hnRNP complexes and serine/arginine-rich splicing factors to influence pre-mRNA splicing (5-7). SAFB1 is also capable of helping in the DNA damage response by allowing yH2AX spreading (8). Knockdown of SAFB1 results in increased SAFB2 expression, and knockdown of SAFB2 results in increased SAFB1 expression (9). Despite their high homology, SAFB1 and SAFB2 have some non-redundant functions. SAFB1 knockout mice have a high degree of growth and fertility issues, while SAFB2 knockout mice have a normal phenotype but aberrations in Sertoli cells (10). SAFB proteins are expressed lowly in breast cancers with poor prognosis (11). Alternate Names DKFZp779C1727; glutathione S-transferase fusion protein; HAP; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein; HET; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAB-B1; SAF-B; SAF-B1; SAFB; SAFB1; scaffold attachment factor B; Scaffold attachment factor B1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG