CST, 65043T, hnRNP L (E5J7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP L. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, hnRNP L (E5J7B) Rabbit Monoclonal Antibody (CST #65043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity hnRNP L (E5J7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP L protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro314 of human hnRNP L protein. Background Heterogeneous nuclear ribonucleoprotein L (hnRNP L) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP L binds to CA repeat elements to regulate splicing and RNA stability (4-6). Binding of hnRNP L has been shown to occur in response to stress stimuli to regulate conformational change and subsequent translation of mRNAs (7). hnRNPL can promote apoptosis by enhancing translation of p53 mRNA (8) . In the context of tumor progression, hnRNPL has been shown to bind and modify translation of critical regulators of cell cycle and apoptosis (9,10). Alternate Names epididymis secretory sperm binding protein; FLJ35509; Heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; HNRNPL; HNRPL; P/OKcl.14 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60, 66 Source/Isotype: Rabbit IgG