CST, 6505S, CHD7 (D3F5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHD7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHD7 (D3F5) Rabbit Monoclonal Antibody (CST #6505) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHD7 (D3F5) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHD7 protein. This antibody also cross-reacts with proteins of unknown origin at 50-55 kDa. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CHD7 protein. Background CHD7 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by altering the chromatin structure at target genes (1,2). The nine members of the CHD family are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). The CHD proteins can be further divided into three subgroups based on the presence of additional conserved functional domains. CHD7 belongs to the third subgroup of CHD proteins together with CHD6, 8, and 9, which are distinguished by the presence of three conserved region (CR) domains, a switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB (SANT) like domain, two brahma and kismet (BRK) domains, and a DNA binding domain (2). CHD7 regulates embryonic stem cell (ESC) specific gene expression together with ESC master regulators Oct-4, Sox2 and nanog, and is necessary for neural stem cell development and formation of the neural crest (5-7). Research studies have shown that CHD7 mutations are frequently found in patients with CHARGE syndrome (coloboma of the eye, heart defects, atresia of the choanae, retardation of growth/development, genital/urinary abnormalities, and ear abnormalities and deafness) (8). Alternate Names ATP-dependent helicase CHD7; CHARGE association; CHD-7; CHD7; chromodomain helicase DNA binding protein 7; chromodomain helicase DNA binding protein 7 isoform CRA_e; Chromodomain-helicase-DNA-binding protein 7; CRG; FLJ20357; FLJ20361; HH5; IS3; KAL5; KIAA1416 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 336 Source/Isotype: Rabbit IgG