CST, 65084SF, Sox10 (D5V9L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SOX10. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Sox10 (D5V9L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65084) is ready to ship. Product Usage Information This product is the carrier free version of product #89356. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 89356 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Sox10 (D5V9L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Sox10 protein. This antibody also recognizes presumptive sumoylated forms of Sox10 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu249 of human Sox10 protein. Background SRY-box 10 (Sox10) is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of cell fate. Sox10 is an important regulator of neural crest and peripheral nervous system development (1-3). Mutations in the gene are associated with a group of auditory-pigmentary developmental disorders, including Waardenburg and Waardenburg-Shah syndromes (3,4). Research studies suggest an oncogenic role for Sox10 in various tumor types, such as hepatocellular carcinoma and melanoma (5,6). Alternate Names DOM; dominant megacolon, mouse, human homolog of; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-box 10; SRY-box transcription factor 10; SRY-related HMG-box gene 10; Transcription factor SOX-10; WS2E; WS4; WS4C Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG