CST, 65118T, SPTA1 (E3B3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPTA1. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, SPTA1 (E3B3L) Rabbit Monoclonal Antibody (CST #65118) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SPTA1 (E3B3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPTA1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln427 of human SPTA1 protein. Background Spectrin alpha chain, erythrocytic 1 (SPTA1), also known as erythroid alpha-spectrin, is a component of the erythrocyte plasma membrane skeleton along with beta-spectrin and protein 4.1. SPTA1 constitutes the lateral linkage in the membrane complex (1). Defects and dysfunction of SPTA1 can contribute to hereditary elliptocytosis (HE), hereditary poikilocytosis (HP), and hereditary spherocytosis (HS), which result in defects in red blood cell morphology and function (1,2). Alternate Names alpha-I spectrin; EL2; elliptocytosis 2; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha chain, erythrocyte; Spectrin alpha chain, erythrocytic 1; spectrin alpha, erythrocytic 1; spectrin, alpha, erythrocytic 1; spectrin, alpha, erythrocytic 1 (elliptocytosis 2); SPH3; SPTA; SPTA1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60-100, 140, 160, 260, 280 Source/Isotype: Rabbit IgG