CST, 65123T, TRBC/TCR beta (E9I1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCRbeta/TCRbeta2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRBC/TCR beta (E9I1S) Rabbit Monoclonal Antibody (CST #65123) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRBC/TCR beta (E9I1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRBC/TCRβ protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with human TRBC/TCRβ recombinant protein. Background The αβ T Cell Receptor (TCR) is a heterodimer composed of an alpha chain and a beta chain expressed on the surface of T cells (1,2). It detects peptide antigen presented by major histocompatibility complex (MHC) molecules (1,2). Detection of cognate antigen leads to activation of the TCR signaling pathway through the CD3 signaling chains that associate with the TCR (1). The TCR chains are made up of a constant region and variable regions that get rearranged during T cell development in the thymus (3). There are two TCRβ constant genes, and (3). A population of normal T cells is polyclonal and contains a mix of TRBC1- and TRBC2-expressing T cells, while T cell malignancies are monoclonal and all express the same TCR which either contains TRBC1 or TRBC2 (4). Therefore, it is possible that T cell malignancies could be identified and targeted with reagents or therapies that can distinguish between TRBC1 and TRBC2 (4). Alternate Names BV05S1J2.2; MGC88817; T cell receptor beta constant 1; T cell receptor beta constant 2; T-cell receptor beta-1 chain C region; T-cell receptor beta-2 chain C region; TCR-beta1; TCR-beta2; TCRB; TCRBC1; TCRBC2; TCRbeta; TCRbeta1; TCRbeta2; TRBC1; TRBC2; V_segment translation product Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30-50 Source/Isotype: Rabbit IgG