CST, 65127T, WHSC1 (D4Z8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WHSC1. Validated for Western Blotting,Immunoprecipitation,CUT&RUN. Available in 2 sizes. Highly specific and rigorously validated in-house, WHSC1 (D4Z8Q) Rabbit Monoclonal Antibody (CST #65127) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:200 CUT&RUN: 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, CUT&RUN Specificity / Sensitivity WHSC1 (D4Z8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total WHSC1 protein. Based on antigen sequence, this antibody should detect WHSC1 Isoform II (UniProt O96028-1) and REIIBP (UniProt O96028-4). This antibody does not detect Isoform I (UniProt O96028-3). Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human WHSC1 protein. Background Wolf-Hirschhorn syndrome candidate protein 1 (WHSC1), also known as Nuclear SET domain-containing protein 2 (NSD2) and Multiple myeloma SET domain-containing protein (MMSET), is a histone methyltransferase protein that catalyzes the di-methylation of histone H3 Lys36, a mark that is associated with transcriptionally active regions of the genome (1,2). WHSC1 haploinsufficiency is implicated in the developmental disorder known as Wolf-Hirschhorn syndrome (WHS), which is characterized by growth and mental retardation, and congenital heart defects (3). In addition, WHSC1 is overexpressed and associated with poor prognosis in a large variety of human cancers, including neuroblastoma, and ovarian, hepatocellular, endometrial, and colorectal carcinoma (4-8). In multiple myeloma, 15-20% of all patients show overexpression of WHSC1 due to a t(4;14)(p16.3;q32) chromosomal translocation that places the gene upstream of a strong enhancer (2). The gene encodes for three major protein isoforms. Isoform II is the full-length protein containing 1365 amino acids. Isoform I, which is generated by alternative splicing, is identical to the N-terminal 647 amino acids of isoform II. A third isoform, known as REIIBP, is generated from a transcript initiating in intron 9 of the WHSC1 locus and is identical to the C-terminal 584 amino acids of isoform II. Alternate Names FLJ23286; Histone-lysine N-methyltransferase NSD2; IL5 promoter REII region-binding protein; KIAA1090; KMT3F; KMT3G; MGC176638; MMSET; multiple myeloma SET domain containing protein type III; Multiple myeloma SET domain-containing protein; NSD2; nuclear receptor binding SET domain protein 2; Nuclear SET domain-containing protein 2; Probable histone-lysine N-methyltransferase NSD2; Protein trithorax-5; REIIBP; trithorax/ash1-related protein 5; TRX5; WHS; WHSC1; Wolf-Hirschhorn syndrome candidate 1; Wolf-Hirschhorn syndrome candidate 1 protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 66, 152 Source/Isotype: Rabbit IgG