CST, 65130T, HOXB7 (E3H4S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HOXB7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HOXB7 (E3H4S) Rabbit Monoclonal Antibody (CST #65130) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity HOXB7 (E3H4S) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOXB7 protein. This antibody also detects a 20 kDa band of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human HOXB7 protein. Background Homeobox (HOX) proteins are homeodomain transcription factors (1). They bind to sequence-specific DNA motifs (C/G)TAATTG and function as master transcriptional regulators, controlling the coordinated expression of genes involved in embryogenesis and tissue differentiation (1,2). HOXB7 belongs to the B group of HOX proteins and plays a dominant role in facilitating tumor progression in many solid tumors. Overexpression of HOXB7 is found in many types of tumors, including melanoma, ovarian, GI-tract, pancreatic ductal adenocarcinoma (PDAC), lung, gliomas, and breast cancers. Aberrant high expression of HOXB7 is associated with poor prognosis (3). The protein promotes tumor cell proliferation, angiogenesis, epithelial-mesenchymal transition, migration, and invasion, as well as drug resistance through regulation of multiple signaling pathways, such as MAPK, VEGF, FGF, TGF-β, and EGFR/Her2 (4-8). HOXB7 binding to a DNA promoter requires its interaction with a cofactor protein such as PBX2 or CBP for transcriptional activation of downstream targets (9,10). Disruption of the HOXB7/PBX complex formation by antagonist peptide HXR9 can inhibit HOXB7 function and is considered as a potential therapeutic value (11). Alternate Names HHO.C1; homeo box 2C; homeo box B7; homeo box c1 protein; homeobox B7; Homeobox protein HHO.C1; Homeobox protein Hox-2C; Homeobox protein Hox-B7; Hox-2.3; HOX2; HOX2C; HOXB7; HXB7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG