CST, 65164S, Tryptase (10D11) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Tryptase. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Tryptase (10D11) Mouse Monoclonal Antibody (CST #65164) is ready to ship. Product Usage Information IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Tryptase (10D11) Mouse Monoclonal Antibody recognizes endogenous levels of tryptase protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to the unbound region of human mast cell tryptase protein. Background Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells (1). Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent) (2). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin (3-5). Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides (6), calcitonin gene-related peptides (7), fibronectins (8), fibrinogens (3), and kininogens (9). Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts (10-13). Alternate Names epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; TPS1; TPS2; TPSAB1; TPSB1; TPSB2; TRYA1; TRYB1; tryptase alpha II; Tryptase alpha-1; tryptase alpha/beta 1; Tryptase alpha/beta-1; tryptase beta 1; tryptase beta I; tryptase beta-1; Tryptase beta-2; tryptase I; Tryptase II; Tryptase-1; Tryptase-2; tryptase-I; tryptase-III Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2b kappa