CST, 65169T, Endophilin-1 (E1E6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying endophilin 1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Endophilin-1 (E1E6Q) Rabbit Monoclonal Antibody (CST #65169) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Endophilin-1 (E1E6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total endophilin-1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly268 of human endophilin-1 protein. Background Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling (1). The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2 (2). Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes (2). Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes (3) and participate in the morphogenesis of dendritic spines (4). Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway (5). Alternate Names bA335L15.1 (SH3-domain GRB2-like 2); CNSA2; EEN-B1; endophilin A1; Endophilin A1 BAR domain; Endophilin-1; Endophilin-A1; FLJ20276; FLJ25015; SH3 domain containing GRB2 like 2, endophilin A1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3-domain GRB2-like 2; SH3D2A; SH3G2; SH3GL2; SH3P4 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG