CST, 65171T, GREB1 (E8M3F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GREB1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GREB1 (E8M3F) Rabbit Monoclonal Antibody (CST #65171) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GREB1 (E8M3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total GREB1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro242 of human GREB1 protein. Background GREB1 was first identified as a gene with a strong response to estrogen receptor (ER) activity (1,2). It has been shown to bind chromatin as an ER-coactivator where it stabilizes interactions with other ER-related cofactors (3). In tamoxifen resistance, Ezh2 represses expression of GREB1, which retargeted ER coregulators (4). In addition to having numerous implications in ER-based pathologies, GREB1 expression has also been found in prostate cancer, where it is regulated by androgen (5). Similarly to breast cancer, GREB1 seems to function as a tumor suppressor and its expression has been found to be correlated with a more favorable prognosis (6). However, GREB1 has also been shown to amplify androgen receptor (AR) output, resulting in enzalutamide resistance (7). Alternate Names gene regulated by estrogen in breast cancer; gene regulated by estrogen in breast cancer protein; Gene regulated in breast cancer 1 protein; GREB1; growth regulating estrogen receptor binding 1; growth regulation by estrogen in breast cancer 1; KIAA0575; Protein GREB1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 216 Source/Isotype: Rabbit IgG