CST, 65237T, TSPAN8 (F8Z7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TSPAN8. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TSPAN8 (F8Z7B) Rabbit Monoclonal Antibody (CST #65237) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TSPAN8 (F8Z7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total TSPAN8 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TSPAN8 protein. Background Tetraspanin 8 (TSPAN8) belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1,2). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs) where they mediate a range of processes, including adhesion, motility, membrane organization, and signal transduction (2,3). TSPAN8 is overexpressed in numerous epithelial cancers, where it promotes cell invasion and metastasis and is associated with poor clinical outcomes (4-6). TSPAN8 also contributes to stemness in cancer stem cells (CSCs) through activation of β-catenin and Sonic hedgehog (Shh) signaling pathways (7,8). CSCs further secrete TSPAN8 on the surface of exosomes, enhancing stemness in the surrounding healthy tissue (9). To this end, TSPAN8 may serve as a useful blood biomarker for the early detection of cancer cells or CSCs (10,11). Therapeutics targeting TSPAN8 have also shown initial success in treating cancer, including monoclonal antibodies, bispecific antibodies, and chimeric antigen receptor (CAR) T cells (12-14). Alternate Names CO-029; tetraspanin 8; Tetraspanin-8; TM4SF3; Transmembrane 4 superfamily member 3; TSN8; Tspan-8; TSPAN8; Tumor-associated antigen CO-029 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG