CST, 65255T, TRIAD3 (F1Z2P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIAD3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIAD3 (F1Z2P) Rabbit Monoclonal Antibody (CST #65255) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRIAD3 (F1Z2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIAD3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys100 of human TRIAD3 protein. Background The role of the innate immune response is to limit the spread of infectious pathogens. Toll-like receptor (TLR) activation results in pro-inflammatory responses that combat infection (1-4). Although TLR signaling generates inflammation to help clear infection, this process can also damage host tissues. It is, therefore, important for cells to have strictly regulated TLR responses. TLRs contain a cytoplasmic Toll/Interleukin-1 receptor (TIR) domain, which is necessary for recruiting proteins involved in regulating TLR signaling. E3 ubiquitin-protein ligase RNF216 (TRIAD3) was first discovered by utilizing a yeast two-hybrid screen (1-4); it was shown that TIR domains of multiple TLRs (TLR3, TLR4, TLR5, and TLR9) bind TRIAD3 (1). TRIAD3 is a ubiquitin-protein ligase that promotes ubiquitination and proteolytic degradation of multiple TLRs, acting as a negative regulator of their signaling activation (1-4). In addition, TRIAD3 interacts and promotes downregulation of two TIR domain-containing adapter molecules, TIR domain-containing adapter-inducing IFN-β (TRIF) and TRIF-related TIR domain-containing adapter protein (TIRAP) (4). Moreover, TRIAD3 has been shown to act as a negative regulator of TNF-α signaling via its interaction with RIP1, effectively impeding RIP1-mediated NF-κB activation (5). TRIAD3 has recently been shown to play a role in slowing cardiac hypertrophy by catalyzing ubiquitination-mediated degradation of TLR4 and TLR9, leading to reduced NF-κB pathway activation (6). TRIAD3 has also been shown to mediate autophagy in neurodegenerative diseases. (5,7). studies show that TRIAD3 decreases the accumulation of phosphorylated tau protein in mouse models. Mutations in lead to increased accumulation of phosphorylated tau and neurodegenerative disease (7). Alternate Names CAHH; E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; RN216; RNF216; Triad domain-containing protein 3; TRIAD3; U7I1; UBCE7IP1; ubiquitin conjugating enzyme 7 interacting protein 1; Ubiquitin-conjugating enzyme 7-interacting protein 1; ZIN; Zinc finger protein inhibiting NF-kappa-B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG