CST, 65344T, UBR5 (D6O8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBR5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UBR5 (D6O8Z) Rabbit Monoclonal Antibody (CST #65344) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBR5 (D6O8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBR5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBR5 protein. Background Human E3 identified by differential display (UBR5/EDD) is a HECT domain-containing ubiquitin E3 ligase of the N-end rule pathway that promotes the ubiquitination and proteasomal degradation of proteins harboring N-degrons (1-3). UBR5 represents an ortholog of HYD, the hyperplastic discs tumor suppressor gene product but has been found to be overexpressed in breast and ovarian cancers, suggesting a possible role in promoting tumor development (4,5). Research studies have demonstrated that UBR5 is functional within the nucleus as it participates in DNA damage signaling by controlling the activities of Chk2, TopBP1, and RNF168 (6-9). Recently, UBR5 was shown to play a novel role in immune cell function by regulating RORγt stability and IL-17 production by Th17 cells (10). Alternate Names DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; EDD; EDD1; FLJ11310; HECT-type E3 ubiquitin transferase UBR5; hHYD; HYD; Hyperplastic discs protein homolog; KIAA0896; MGC57263; progestin induced protein; Progestin-induced protein; ubiquitin protein ligase E3 component n-recognin 5; ubiquitin-protein ligase; UBR5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG