CST, 65419SF, SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Spike Protein SARS-CoV-2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65419) is ready to ship. Product Usage Information This product is the carrier free version of product #99423. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 99423 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SARS-CoV-2 spike protein. This antibody detects full-length SARS-CoV-2 spike protein and also detects the S1 domain generated by furin cleavage. This antibody has been confirmed to detect the following SARS-CoV-2 isolates and variants: USA-WA1/2020, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (B.1.1.529). This antibody does not cross-react with spike proteins from SARS or MERS coronaviruses. Non-specific nuclear foci staining was observed in some mouse tissues by immunohistochemistry. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the S1 domain of SARS-CoV-2 spike protein (USA-WA1/2020 isolate). Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2' sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9). Alternate Names 2019-nCoV surface glycoprotein; Peplomer protein; S glycoprotein; SARS-CoV-2 spike protein; spike glycoprotein; SPIKE_SARS2; surface glycoprotein Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 110, 220 Source/Isotype: Rabbit IgG