CST, 65494SF, MHC Class I (E8E7N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying H2-D1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MHC Class I (E8E7N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65494) is ready to ship. Product Usage Information This product is the carrier free version of product #76828. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 76828 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MHC Class I (E8E7N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MHC Class I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu254 of mouse H2-D1 protein. Background The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8 T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4 T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2). Alternate Names H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H-2D cell surface glycoprotein; H-2D(B); H2-D; H2-D1; H2-DC1-beta transplantation antigen protein; H2-K1; HA11; histocompatibility 2, D region locus 1; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG