CST, 65534S, CD10/Neprilysin (E5P7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD10. Validated for WB,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD10/Neprilysin (E5P7S) Rabbit Monoclonal Antibody (CST #65534) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:250 - 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 54370 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CD10/Neprilysin (E5P7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD10/Neprilysin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD10/Neprilysin recombinant protein. Background CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4). Alternate Names Atriopeptidase; CALLA; CD10; CMT2T; Common acute lymphocytic leukemia antigen; DKFZp686O16152; Enkephalinase; EPN; membrane metallo-endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; membrane metalloendopeptidase; MGC126681; MGC126707; MME; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase; Neutral endopeptidase 24.11; SCA43; SFE; Skin fibroblast elastase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG