CST, 65552S, L-asparaginase/ASRGL1 Antibody

Polyclonal Antibody for studying ASRGL1. Validated for Western Blotting. Highly specific and rigorously validated in-house, L-asparaginase/ASRGL1 Antibody (CST #65552) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity L-asparaginase/ASRGL1 Antibody recognizes endogenous levels of total L-asparaginase/ASRGL1 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human L-asparaginase/ASRGL1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background L-asparaginase (ASRGL1) catalyzes the conversion of L-asparagine to L-aspartate. Research studies have shown that intracellular asparagine can suppress apoptosis in a large number of human tumors (1). In addition, acute lymphocytic leukemia cells frequently depend upon serum asparagine for their viability, as they lack asparagine synthetase (ASNS). Deprivation of asparagine by L-asparaginase has therefore been developed as a therapeutic treatment for acute lymphocytic leukemia (2-3). In mutant non-small cell lung carcinoma (NSCLC) cells, PI3K/Akt signaling was shown to be required for expression, suggesting combinatorial Akt inhibition and L-asparaginase treatment as a therapeutic strategy for NSCLC (3). Research studies on a breast cancer model have furthermore shown that restriction of asparagine can suppress cancer metastasis (4). Alternate Names ALP; ALP1; ASGL1; asparaginase and isoaspartyl peptidase 1; asparaginase like 1; asparaginase-like 1 protein; Asparaginase-like protein 1; ASRGL1; Beta-aspartyl-peptidase; CRASH; FLJ22316; Isoaspartyl dipeptidase; Isoaspartyl peptidase/L-asparaginase; Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain; L-asparaginase; L-asparagine amidohydrolase; testis secretory sperm-binding protein Li 242mP Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 41 SOURCE: Rabbit